tetanus toxin light chain telc sequence Search Results


200 500 n m tetrodotoxin  (Alomone Labs)
94
Alomone Labs 200 500 n m tetrodotoxin
200 500 N M Tetrodotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
200 500 n m tetrodotoxin - by Bioz Stars, 2026-03
94/100 stars
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botulinum toxin type a  (Merz Pharmaceuticals)
90
Merz Pharmaceuticals botulinum toxin type a
Botulinum Toxin Type A, supplied by Merz Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
botulinum toxin type a - by Bioz Stars, 2026-03
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tetanus toxin light chain telc sequence  (Addgene inc)
93
Addgene inc tetanus toxin light chain telc sequence
( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by <t>TeLC-dependent</t> cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices <t>expressing</t> <t>Syn-mCherry</t> (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .
Tetanus Toxin Light Chain Telc Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
tetanus toxin light chain telc sequence - by Bioz Stars, 2026-03
93/100 stars
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dmem/f12  (Welgene inc)
90
Welgene inc dmem/f12
( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by <t>TeLC-dependent</t> cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices <t>expressing</t> <t>Syn-mCherry</t> (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .
Dmem/F12, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem/f12/product/Welgene inc
Average 90 stars, based on 1 article reviews
dmem/f12 - by Bioz Stars, 2026-03
90/100 stars
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pgj 2  (Cayman Chemical)
90
Cayman Chemical pgj 2
( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by <t>TeLC-dependent</t> cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices <t>expressing</t> <t>Syn-mCherry</t> (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .
Pgj 2, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgj 2/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
pgj 2 - by Bioz Stars, 2026-03
90/100 stars
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cholera toxin  (Millipore)
90
Millipore cholera toxin
( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by <t>TeLC-dependent</t> cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices <t>expressing</t> <t>Syn-mCherry</t> (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .
Cholera Toxin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cholera toxin/product/Millipore
Average 90 stars, based on 1 article reviews
cholera toxin - by Bioz Stars, 2026-03
90/100 stars
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for toxic substances and disease registry [atsdr]  (Disease Registry)
90
Disease Registry for toxic substances and disease registry [atsdr]
( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by <t>TeLC-dependent</t> cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices <t>expressing</t> <t>Syn-mCherry</t> (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .
For Toxic Substances And Disease Registry [Atsdr], supplied by Disease Registry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/for toxic substances and disease registry [atsdr]/product/Disease Registry
Average 90 stars, based on 1 article reviews
for toxic substances and disease registry [atsdr] - by Bioz Stars, 2026-03
90/100 stars
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mgtx  (Alomone Labs)
93
Alomone Labs mgtx
( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by <t>TeLC-dependent</t> cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices <t>expressing</t> <t>Syn-mCherry</t> (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .
Mgtx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgtx/product/Alomone Labs
Average 93 stars, based on 1 article reviews
mgtx - by Bioz Stars, 2026-03
93/100 stars
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bordetella pertussis toxin antibody (1280/204) - bsa free  (Novus Biologicals)
N/A
The Bordetella Pertussis Toxin Antibody (1280/204) - BSA Free from Novus is a Bordetella Pertussis Toxin antibody to Bordetella Pertussis Toxin. This antibody reacts with Bacteria. The Bordetella Pertussis Toxin antibody has been validated for
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adenylate cyclase toxin from bordetella  (Cayman Chemical)
N/A
Adenylate Cyclase Toxin from Bordetella pertussis recombinant frozen liquid
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bordetella pertussis toxin igg negative human plasma  (Biosynth Carbosynth)
N/A
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bordetella pertussis toxin antibody (1280/204) [dylight 650]  (Novus Biologicals)
N/A
The Bordetella Pertussis Toxin Antibody (1280/204) [DyLight 650] from Novus is a Bordetella Pertussis Toxin antibody to Bordetella Pertussis Toxin. This antibody reacts with Bacteria. The Bordetella Pertussis Toxin antibody has been validated for the
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Image Search Results


( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by TeLC-dependent cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices expressing Syn-mCherry (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .

Journal: The EMBO Journal

Article Title: Rhythmic astrocytic GABA production synchronizes neuronal circadian timekeeping in the suprachiasmatic nucleus

doi: 10.1038/s44318-024-00324-w

Figure Lengend Snippet: ( A ) Schematic showing mechanism of blockade of GABAergic synaptic transmission by TeLC-dependent cleavage of the SNARE complex protein VAMP2. ( B ) Representative widefield images and insets of SCN slices expressing Syn-mCherry (control, top) or Syn-TeLC-mCherry (bottom) labeled with anti-VAMP2 antibody and DAPI, scale bar = 200 µm. Inset scale bar = 20 µm. ( C ) Quantification of mean VAMP2 intensity within the SCN. N Syn-mCh = 3, N Syn-TeLC = 3 SCN slices, two-tailed t test, P = 0.0008. ( D ) Representative detrended time series of SCN slices expressing reporters for extracellular GABA (Syn-GABASnFR) and PER2::LUC before and after treatment with Syn-mCherry (left) or Syn-TeLC-mCherry (right). ( E ) Amplitude relative to baseline of rhythms of PER2::LUC (left) and Syn-GABASnFR (right). Two-way ANOVA with post hoc Šidák’s test, PER2::LUC: P = 0.0152 for baseline-Day 1–6; P = 0.0002 for baseline->7 d after; Syn-GABASnFR: P = 0.042 for BSL- > 7 d after, P = 0.0249 for Day 1-6- > 7 d after. ( F ) Circadian period of PER2::LUC (left) and Syn-GABASnFR rhythms (right). N PER2::LUC = 5 SCN slices, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( G ) Mean fluorescence intensity of Syn-GABASnFR signal across timepoints. N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. Two-way ANOVA with post hoc Šidák’s test shown. ( H ) Representative circadian phase cluster map of PER2::LUC before and >7 days after transduction with Syn-TeLC-mCherry (bottom) or mCherry control (top). One SCN nucleus is shown, orientation as indicated (dorsal—D, medial—M). Color bar indicates circadian phases of clusters, NR = non-rhythmic. White vector indicates the directionality of phase progression. ( I ) Representative standard deviation of cluster time series shown in ( H ). ( J ) Representative circadian phase cluster map of Syn-GABASnFR co-detected with PER2::LUC shown in ( H ) before and >7 days after viral transduction. ( K ) Representative standard deviation of cluster time series shown in ( J ). ( L ) Inter-cluster phase dispersal of PER2::LUC (top) or Syn-GABASnFR (bottom) relative to baseline, with two-tailed t test, PER2::LUC P = 0.0338, Syn-GABASnFR P = 0.259; N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. ( M ) PDF of cluster phase variance shown in ( L ); N PER2::LUC = 5, N Syn-GABASnFR = 8 SCN slices. All data shown mean ± SEM unless otherwise indicated. For longitudinal data, connecting lines are shown between means. ns = non-significant. * P < 0.05, *** P < 0.001. A detailed statistical report for this figure is provided in Appendix Table . .

Article Snippet: Syn1-TeLC-P2A-mCherry-WPRE was designed and purchased from Vectorbuilder with tetanus toxin light chain (TeLC) sequence from Addgene plasmid #159102 (Sandeep Datta).

Techniques: Transmission Assay, Expressing, Control, Labeling, Two Tailed Test, Fluorescence, Transduction, Plasmid Preparation, Standard Deviation

Reagents and tools table

Journal: The EMBO Journal

Article Title: Rhythmic astrocytic GABA production synchronizes neuronal circadian timekeeping in the suprachiasmatic nucleus

doi: 10.1038/s44318-024-00324-w

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Syn1-TeLC-P2A-mCherry-WPRE was designed and purchased from Vectorbuilder with tetanus toxin light chain (TeLC) sequence from Addgene plasmid #159102 (Sandeep Datta).

Techniques: Recombinant, Plasmid Preparation, Software